Peroxidase and fluorescein isothiocyanate as antibody markers. A quantitative comparison of two peroxidase conjugates prepared with glutaraldehyde or periodate anda fluorescein conjugate.
نویسندگان
چکیده
منابع مشابه
comparison of the photobleaching and photostability traits of alexa fluor 568- and fluorescein isothiocyanate- conjugated antibody
objective: synthetic fluorescent dyes that are conjugated to antibodies are useful tools to probe molecules. based on dye chemical structures, their photobleaching and photostability indices are quite diverse. it is generally believed that among different fluorescent dyes, alexa fluor family has greater photostability than traditional dyes like fluorescein isothiocyanate (fitc) and cy5. alexa f...
متن کاملRapid fluorescein and protein assay method for fluorescent-antibody conjugates.
A method is presented for the rapid determination of the fluorescein content, protein content, and fluorescein-to-protein ratio for immune globulin conjugates with fluorescein isothiocyanate as the fluor. This method is based on the absorbance of the fluorescent antibody at those wavelengths primarily associated with the fluorescein and gamma-globulin fractions, and permits these materials to b...
متن کاملComparison of the Photobleaching and Photostability Traits of Alexa Fluor 568- and Fluorescein Isothiocyanate- conjugated Antibody
OBJECTIVE Synthetic fluorescent dyes that are conjugated to antibodies are useful tools to probe molecules. Based on dye chemical structures, their photobleaching and photostability indices are quite diverse. It is generally believed that among different fluorescent dyes, Alexa Fluor family has greater photostability than traditional dyes like fluorescein isothiocyanate (FITC) and Cy5. Alexa Fl...
متن کاملPolyelectrolyte Multilayer Microcapsules Containing Fluorescein Isothiocyanate- Concanavalin A/Glycogen Conjugates for Fluorometric Determination of Sugars
Polyelectrolyte multilayer microcapsules were prepared by a layer-by-layer deposition of poly(ethyleneimine) (PEI) or poly(allylamine hydrochloride) (PAH) and poly(styrene sulfonate) (PSS) on a calcium carbonate particle containing fluorescein isothiocyanateconcanavalin A (FITC-Con A)/glycogen conjugates. Fluorescence microscope and scanning electron microscope observations revealed that the mi...
متن کاملHoney-Induced Protein Stabilization as Studied by Fluorescein Isothiocyanate Fluorescence
Protein stabilizing potential of honey was studied on a model protein, bovine serum albumin (BSA), using extrinsic fluorescence of fluorescein isothiocyanate (FITC) as the probe. BSA was labelled with FITC using chemical coupling, and urea and thermal denaturation studies were performed on FITC-labelled BSA (FITC-BSA) both in the absence and presence of 10% and 20% (w/v) honey using FITC fluore...
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ژورنال
عنوان ژورنال: Journal of Histochemistry & Cytochemistry
سال: 1976
ISSN: 0022-1554,1551-5044
DOI: 10.1177/24.9.184204